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TCMK-1 ATCC细胞购买

  • 更新时间:  2019-04-17
  • 产品型号:  ATCCCCL-139
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  国家标准物质、国内外标准品、对照品、实验耗材、试剂、菌株、细胞、ATCC、培养基、血清、冶金标样、标准气体、试剂盒等,欢迎查询!

ATCCCCL-139 TCMK-1 ATCC细胞购买

基本信息
资源编号ATCCCCL-139
资源名称TCMK-1
种属Mus musculus, mouse kidney
类型SV40 transformed
形态epithelial
提供形式frozen
致病类型normal
安全等级2
培养方法
培养基The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
传代方法Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
生长条件Temperature: 37°C
生长特性adherent
存储条件liquid nitrogen vapor phase;Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
详细说明
描述About 2% of the cells are positive for SV40 viral antigen, but virus is not recoverable.
Tested and found negative for ectromelia virus (mousepox).
Ageweanling
StrainC3H/Mai
Karyotypemodal number = 78; range = 64 to 157.All cells showed 2-6 acrocentric chromosomes with secondary constrictions and 1-4 biarmed chromosomes per cells. Sixty percent of the cells had one or more minute chromosomes.
DerivationThe cells are SV40 transformed and are positive for SV40 T-antigen.
Antigen ExpressionH-2k
TumorigenicNo
EffectsNo, in immunosuppressed mice
Yes, in semisolid medium
Virus SusceptibilityVesicular stomatitis, Glasgow (Indiana) Human poliovirus 1
Virus Resistancepoliovirus 1
Year of Origin1963

ATCCCCL-139 TCMK-1 ATCC细胞购买

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